![]() While there have been ample studies to justify the use of antibiotics in traumatic eye injury, the effects of retinal lesions with the treatment of traditional medicine were largely unknown. ![]() While there were many clinical examples of ocular trauma, the histopathology of retinal responses was not properly documented, and research on animal models was scanty. ![]() Present treatments of ocular trauma included antibiotics, steroids, and surgical repair. With retinal involvement, an additional 0.2% of patients developed sympathetic ophthalmia. Penetrating and global injuries were the most serious, as they could damage the lens, iris, and vitreous, and result in retinal or choroidal detachment. Apart from designating the injury as close or open, the classification of global, adnexal, or mechanical injury could also be used. Adults might also be affected, especially young males of 18 - 24 years old, usually as a result of injury from sport, assault or accidents. Ocular trauma is an eye problem resulting from injury, and 40% of monocular traumas usually come from children at school or at home. Conclusion: Angong Niuhuang pill is a traditional medicine composite formula with antibiotic and neuroprotective effects on the brain and the eyes, and this work also pointed out its possible pharmaceutical potential. Catalase reactions confirmed equivalent findings that the treated retinae with chloramphenicol or Angong treatment had less catalase. In the chloramphenicol and Angong groups, some cells in the outer nuclear layer displayed BDNF. BDNF was in the inner nuclear layer and ganglion cell layer of all groups. Result: It was evident that both the retinae treated with chloramphenicol and Angong had fewer apoptotic cells and more proliferative cells, particularly in the cases of the Angong treatment. The whole experiment was repeated two times. Immunohistochemical evaluation of proliferative cell nuclear antigen (PCNA), brain-derived neurotrophic factor (BDNF), catalase, and cleaved caspase-3, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) for apoptotic cells were performed on the fish’s superior quadrants of the retinae. Angong after seven days, the animals were sacrificed. The animals were divided into three groups and received treatments of saline, chloramphenicol, and Angong Niuhuang pill (Angong) eyedrops twice a day respectively. Materials and Methods: The penetration of the eyes was performed at 2mm above the midline of the limbus in the superior quadrant with an 18-gauge sterile needle, all the way to the retina but avoiding the lens while the animals were anaesthetized in water containing 100 mg/L ethyl 3-aminobenzoate methanesulfonate (MS-222). In this work, goldfish was employed as a model for penetrating injury of the eyes to study the ocular trauma with antibiotic and traditional medicine treatments. The consequences of retinal lesions with traditional medicine treatment were mostly unknown. Purpose: While there were numerous clinical examples of ocular damage, the histopathology of retinal responses was poorly characterized, and animal model research was limited.
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